2 edition of regulation of glutamic acid metabolism during sporogenesis of Bacillus cereus t. found in the catalog.
regulation of glutamic acid metabolism during sporogenesis of Bacillus cereus t.
William Peter Weisrock
Written in English
|The Physical Object|
|Pagination||ix, 71 l.|
|Number of Pages||71|
Poly-gamma-glutamic acid (γ-PGA) is a promising macromolecule with potential as a replacement for chemosynthetic polymers. γ-PGA can be produced by many microorganisms, including Bacillus species. Bacillus licheniformis CGMCC secretes γ-PGA when using glycerol and trisodium citrate as its optimal carbon sources and secretes Cited by: 8. The B. cereus strains, the Serratia marcescens strain and the Arthrobacter sp. grew well on a mineral salts α-cellulose agar. One of the Bacillus cereus strains and Serratia marcescens hydrolysed gels of carboxymethylcellulose. All isolates .
To study the effect of Ca2+ on glutamate dehydrogenase (GDH) and its role in poly-γ-glutamic acid (γ-PGA) synthesis in Bacillus natto HSF When the concentration of Ca2+ varied from 0 to g/l in the growth medium of B. natto HSF , γ-PGA production increased from to g/l, while GDH specific activity and NH4Cl consumption improved Cited by: 8. Bacillus subtilis UPMB13 was found to be an L-glutamic acid independent producer of extracellular polymeric substances (EPS) with bioflocculation properties. Optimum production of the bioflocculant was found to be at the early stage of cell propagation of 24–72 h of fermentation. At a limited nutrient input of mL tryptic soy broth, the flocculating activities Cited by: 1.
Poly-γ-glutamic acid (γ-PGA) is a valuable polymer with glutamate as its sole precursor. Enhancement of the intracellular glutamate synthesis is a very important strategy for the improvement of γ-PGA production, especially for those glutamate-independent γ-PGA producing strains. Corynebacterium glutamicum has long been used for industrial glutamate Cited by: 4. The Bacillus cereus group includes several Bacillus species with closely related phylogeny. The most well-studied members of the group, B. anthracis, B. cereus, and B. thuringiensis, are known for their pathogenic potential. Here, we present the historical rationale for speciation and discuss shared and unique features of these by:
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Bacillus cereus T, sporulating in a chemically defined medium under optimum conditions, requires substrate quantities of glutamate during the first 4 h of sporogenesis.
Seventy percent of the glutamate utilized was catabolized to CO2 during this period, with the remaining glutamate carbon assimilated into various spore constituents, principally Cited by: 8. —Growth and sporulation were studied in Bacillus cereus by use of an active culture technique and a synthetic medium.
A high level of glutamic acid (70 mm) was required for optimal growth and glucose oxidation followed by sporulation even though relatively little glutamic acid was consumed (14 mm).Cited by: been placed on the investigation of pathways related to the metabolism of glutamic acid, and to some extent glucose, and an evaluation of the degree of prominence these pathways might play in growth and sporulation of Bacillus cereus var.
mycoides. In Bacillus thuringiensis, a close relative of B. cereus (21), glutamate appears to regulate TCA cycle activity as well as influence spore heat resistance and DPA content (35). cereus has been reported to metabolize glutamate primarily as an energy source during the time of sporogenesis (10).Cited by: Abstract.
Nakata, H. (Washington State University, Pullman). Role of acetate in sporogenesis of Bacillus cereus.J. Bacteriol. – —The distribution of radioactivity associated initially with acetateC 14 was followed during sporogenesis of Bacillus cereus strain T.
This was accomplished by replacing cells committed to sporulation into a chemically defined Cited by: Bacillus cereus T, sporulating in a chemically defined medium under optimum conditions, requires substrate quantities of glutamate during the first 4 h of sporogenesis. HARDWICK WA, FOSTER JW.
Enzymatic changes during sporogenesis in some aerobic bacteria. J Bacteriol. Apr; 65 (4)– [PMC free article] KEYNAN A, STRECKER HJ, WAELSCH H. Glutamine, glutamic acid, and glycolysis in Bacillus subtilis. J Biol Chem. Dec; (2)– THORNE CB, GOMEZ CG, NOYES HE, HOUSEWRIGHT by: 6.
The Gram-positive soil bacterium Bacillus subtilis encounters changing environmental conditions in its habitat. The access to oxygen determines the mode of energy generation.
A complex regulatory network is employed to switch from oxygen respiration to nitrate respiration and various fermentative processes. cells of Clostridium tetuni (2). Glutamine, glutamic acid, and related com- pounds have also been reported to increase aerobic glycolysis in brain slices (3).
During an investigation of the metabolism of glutamine and glutamic acid by Bacillus subtilis it was observed that both of these compounds markedly stimulated the utilization of glucose.
Trophic Level-Bacillus cereus is a heterotrophic feeder. Heterotrophic in Latin translates to hetero= "another" trophic="nutrition".
This means that to be heterotrophic, you feed on other organisms to acquire you nutrition. Also, Bacillus cereus can be classified as a parasitic organism, because it utilizes hosts such as humans and insects.
Nitrogen source regulation of glutamate synthase activity in Bacillus subtilis occurs at the level of transcription of the gltA and gltB genes, which encode the two subunits of the enzyme.
The peptide antibiotics tyrocidine and gramicidin may have a regulatory function during sporogenesis of Bacillus brevis. The investigation of L-glutamic acid fermentation was started in our country in Regulation of intermediary metabolism of Aspergillus niger in relation to citric acid production was studied on a biochemical basis.
It is a simple and effective method to control the production of fermented products by metal ions. In this study, effect of KCl on γ-PGA production by B. subtilis GXA was investigated.
K + was proved to be the true effector, and concentration of mM was optimized for γ-PGA production. Addition of KCl to medium effectively reduced broth viscosity from mPa s Cited by: multiply the number per serving by 9.
divide the number of kcal by 4. divide the number of kcal by 9. divide the number of kcal by 4. Researchers are conducting a study to determine the effects of vitamin D supplements on the adult human immune system.
The study involves providing pills that contain vitamin D to one group of human adults, and. Glutamic acid and alanine comprise at least 60% of the amino acid pool of B. licheniformis A-5, B. subti and B. cereus T during all stages of growth and sporulation.
Yu et al. Microb Cell Fact DOI /s RESEARCH Effect of glucose on poly-γ-glutamic acid metabolism in Bacillus licheniformis Wencheng Yu 1,2†, Zhen Chen1,2†, Hong Ye 1,2, Peize Liu1,2, Zhipeng Li3, Yuanpeng Wang 1,2, Qingbiao Li1,2, Shan Yan 4, Chuan‑jian Zhong4 and Ning He1,2* Abstract Background: Poly‑gamma‑glutamic acid Cited by: 8.
During spore formation, the enzymes involved in the synthesis of such substances as the coat proteins and dipicolinic acid must be formed. The completed spore, which takes several generation times to form and results from complex differentiation process, is remarkably resistant to heat, chemicals, and adverse environmental by: Assessment of the Metabolism of Different Strains of Bacillus megaterium Braz.
Arch. Biol. Technol. v n pp.July/Aug Table 1 - Maximum concentrations of cells (Cx), acetic acid and lactic acid, maximum specific growth rates (µmax). The following types of regulation are assumed to control the synthesis of proteinases in bacilli (3): (i) catabolite re pression, (ii) suppression by growth and derepression in the stationary phase, (iii) triggering and subsequent switching off by the developmental mechanisms during sporogenesis, (iv) repression by amino acids: points (i Cited by: 1.
ABSTRACT. Bacillus megaterium is a promising host for expression of heterologous proteins. This paper reports the nutrient consumption patterns and production of metabolites for three different strains of B. megaterium, ATCCQMB and PVwhich is QMB with seven constitutive plasmids deleted.
14 h cultivations in agitated flasks were run, for two. Poly-γ-glutamic acid (γ-PGA) is a natural biopolymer of glutamic acid. The repeating units of γ-PGA may be derived exclusively from d-glutamic acid, or l-glutamic acid, or both.
Poly-gamma-glutamic acid (γ-PGA) is a type of polyamide composed of single glutamic acids joined via γ-amide linkages between the glutamate γ-carboxyl and α-amino groups .
γ-PGA is a natural macromolecular polymer that is biodegradable, edible andγ-PGA and its derivatives have been applied in diverse fields, particularly as Cited by: 8.
A strain ofBacillus cereus es isolated from Burdwan soil producesl-glutamate in the medium. The strain is able to grow and produce in a synthetic medium but supplementation with casamino acid or yeast extract improves the yield. Maintenance of pH of the fermentation medium near neutrality prolongs the active growth period and improves the yield.
Cited by: 5.